The expected size of the PCR product is 357 + 280 bp, or 637 bp. Primer B-anchored products add 280 bp of CDS to the PCR product. cerevisiae, these regulatory elements are usually located within 1 kb of the CDS.Ī is 357 nucleotides upstream of the SAM1initiation codon (nucleotide 1001). SGD curators generated these records because researchers are often interested in studying regulatory elements that control transcription of a gene and the processing of gene transcripts. These records contain the CDS for your MET gene together with 1 kb of upstream and 1 kb of downstream sequence. To constsruct your map, you can take advantage of special genome sequence records prepared by SGD curators. A PCR product may or may not contain portions of the MET gene’s CDS, depending on whether you are analyzing a strain with the native or disrupted MET gene. All PCR products should contain a portion of the MET gene’s 5’-flanking region because of primer A. To assist those calculations, prepare a simple map of the genomic region containing your gene with the primer sequences aligned against the genome sequence. To interpret those results, it will be important to have calculated the expected sizes of the PCR products from your reactions. In the next lab, you will analyze your PCR reaction products on an agarose gel that separates DNA molecules according to their sizes.

Associate Professor Emeritus (Biology) at Boston College.

Pcr 800 amp